This competitive ELISA kit is for determination of 14,15-DHET levels in biological samples including urine, plasma, cell lines and tissues. 14,15-DHET is a representative metabolite of soluble epoxide hydrolase-mediated metabolism of EETs, which are generated by arachidonic acid epoxygenase activity of cytochromes P450. Our company found that 14,15-DHET level exhibited strong positive correlation with hypertension in rodents and human preeclampsia patients (two US patents and one pending international patent). In addition, we developed other hypertensive ELISA kits to measure the levels of 11,12-DHET and 20-HETE, and new Prostaglandin 8-Isoprostane.Each kit for triplicate analyses of up to 24 samples contains one 96 well plate, one tube of 14,15-DHET standard, one tube of 14,15-DHET-conjugated horseradish peroxidase (HRP), and buffers for sample and HRP dilutions, and plate washing.