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Measuring Activity and Inhibition of Soluble Epoxide Hydrolase (sEH)

Soluble epoxide hydrolase (sEH) is a key enzyme in the arachidonic pathway due to it’s ability to biotransform anti-hypertensive epoxyeicosa-trienoic acids (EETs) to dihydroxyeicosatrienoic acids (DHETs) thereby making soluble epoxide hydrolase an important pharmacological target. 

This colorimetric ELISA assay kit is suitable for measurements of sEH enzyme activity in tissues and cells (cell lysates or media) using the substrate 14,15-EET.. 

Additionally, the assay kit can be used to screen for inhibitors of soluble epoxide hydrolase activity (See experimental results from an AUDA study above)).

Each 96-well plate is suitable for triplicate analyses of up to 24 samples.

Recent sEH inhibitor studies using our SH1 Kit

Zhang, L et al. (2020) Protective effects of the soluble epoxide hydrolase inhibitor 1-trifluoromethoxyphenyl-3-(1-propionylpiperidin-4-yl) urea in a rat model of permanent middle cerebral artery occlusion. Frontiers in Pharmacology 11: 182. PMID: 32184732

Wu, J. et al. (2020) Soluble epoxide hydrolase inhibitor protects against blood-brain barrier dysfunction in a mouse model of type 2 diabetes via the AMPK/HO-1 pathway. Biochem. Biophys. Res. Commun. 524: 354-359. PMID: 32001002

Jiang, J. et al. (2020) Inhibition of soluble epoxide hydrolase attenuates airway remodeling in a chronic asthma model. European Journal of Pharmacology 868: 172874 PMID: 31866410

Chen, Z. et al. (2020) sEH inhibitor TPPU ameliorates cecal ligation and puncture-induced sepsis by egulating macrophage functions. Shock 53: 761-771. PMID: 31318834

Related Products From Detroit R&D: 

14,15-DHET ELISA Cat #DH1  

11,12-DHET ELISA Cat #DH4

sEH antibody Cat #SEH1