The 14,15-DHET is a representative metabolite of soluble epoxide hydrolase (sEH)-mediated metabolism of EETs, generated by arachidonic acid epoxygenase activity of cytochromes P450 (CYPs) 2C and 2J.

This competitive ELISA kit with an HRP system has been used to determine 14,15-DHET levels in biological samples (tissue, plasma and urine) and cell culture media. Using the 14,15-DHET ELISA, a strong positive correlation has been discovered with 14,15-DHET levels in hypertension and brain injury and stroke. In one study, urinary 14,15-DHET levels of pregnancy-induced hypertensive (preeclamptic) women were significantly different at 98% confidence (p